Özet:
In this study, chitosan, a natural cationic polysaccharide obtained by natural
acetylation method, garlic extract garlic and goji beri extract were encapsulated with
taurine extracts. Spray drying method was used in the encapsulation process. Drying
inlet temperature was determined as 120 oC and outlet temperature as 45 oC. The
extracts and encapsulated chitosan compounds were analyzed by Fourier transformed
infrared spectroscopy (FTIR) and High Performance Liquid Chromatography
(HPLC) and their molecular weights were determined by cryoscopy method. The %
antioxidant activity of the synthesized compounds was analyzed by UV
spectrophotometer and the loading capacity of the antioxidant compounds in
encapsulation was calculated. Then, antibacterial analyzes and surface activity
analyzes of the synthesized compounds were performed against Listeria
monocytogenes, Staphylococcus aureus, E.coli and Salmonella bacteria using well
diffusion method and Zeiss microscope. According to the analysis results %
antioxidant activity values of synthesized CSA and CSB compounds were
determined as 49.3 % and 58.7 % respectively. Molecular weights were calculated as
67, 95 and 134 kDa for chitosan (CS), CSA and CSB compounds, respectively. The
loading capacities of the antioxidant compounds in encapsulation were analyzed as
58.4 % and 65.1 % for CSA and CSB, respectively. Four different bacteria were used
for antibacterial analysis of chitosan and encapsulated CSA and CBS compounds.
E.coli and Salmonella bacteria were used as gram negative bacteria, S.aureus and
L.monocytogenes cultures were used as gram positive bacteria. According to the
antibacterial analysis results, the largest inhibition zones for CSA and CS compounds
were obtained against Listeria monocytogenes bacteria. According to the surface
activity analysis results of the compounds against E.coli, Salmonella, Listeria
monocytogenes and S.aureus bacteria, significant surface activity results were
obtained against all bacteria except S.aureus bacteria.