Synthesis of Graphene Oxide and in vitro Evaluation of Its Cytotoxic Effect

Abstract

Objective:Graphen oxide, a carbon-based material, is considered as a potential material for many biomedical applications such as cell labeling, cell imaging, biosensors, drug release studies, due to its physical and chemical properties. Graphene oxide can be synthesized by more than one method. In this study, it was aimed to evaluate the cytotoxic effects of the graphenoxide samples we synthesized. Methods:Synthesis of graphene oxide was done by Hummers method. The cytotoxic effects of graphene oxide were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay in human embryonic kidney cells (HEK293). After 24 hours, 48 hours and 72 hours of graphene oxide incubation at different doses, the IC50 values were calculated which 50% percentage of cell viability. Results:After 24 hours, 48 hours and 72 hours, IC50 values were measured as 206.18 .ig/mL, 108.98 .ig/mL, and 55.54 .ig/mL, respectively. It was determined that the IC50 value decreased as the incubation time increased. Conclusion:It was determined that the cytotoxic effect of the synthesized graphene oxide varies depending on the exposure time.