Abstract:
ABSTRACT
Purpose: The aim of the current study was to establish
protocols for Erbium Chromium-doped Yttrium-Scandium Gallium-Garnet (Er,Cr:YSGG) laser application for
detoxification of implant surface, preservation of surface
biocompatibility and enhancement of osseointegration.
In this purpose, four different variables including power
(W), frequency (Hz), distance (mm) and duration (sn) were
investigated at 3 different levels.
Material and Methods: Grade 5 titanium discs infected by
S.aureus were detoxified with Er, Cr: YSGG laser according
to various protocols. After laser application, surface
morphology and surface roughness of titanium discs as well
as cellular morphology and proliferation of osteoblasts-like
cells at the end of 24 and 48 hours (SaOs-2 cell culture) were
examined.
Results: The most remarkable changes on the surface
of titanium discs were observed in group Test 8 (3 W-25
Hz-2 mm-45 sn) which was exposed to the highest power
density (W/cm2).. In this protocol, melting and flattening
on the surface was observed most prominently and surface
roughness (Ra) was lowest. Proliferation indicators in groups
Test 1 and Test 7 were found to be statistically significantly
increased compared to the control group at the end of 48
hours. Furthermore, Ra values of these 2 groups (Test 1 and
Test 7) were similar to that of control group.
Discussion: To conclude, our results have shown that power
intensity, which is linked with distance, was the leading
parameter for alteration of surface morphology. We suggest
that cellular proliferation during reosseointegration is
facilitated by conditions that maintain surface roughness in
its initial form and amplify surface biocompatibility.
